|
|
Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
23/06/2017 |
Data da última atualização: |
10/07/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CASTRO, V. L. S. S. de; CASTANHA, R. F.; VALLIM, J. H. |
Afiliação: |
VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; RODRIGO FERNANDES CASTANHA, CNPMA; JOSE HENRIQUE VALLIM, CNPMA. |
Título: |
Evaluation of NANoREG TiO2 toxicity in Caenorhabiditis elegans. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B4TJ. |
Idioma: |
Inglês |
Conteúdo: |
Caenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on the concentration tested, but also on the endpoint. Growth seems to be a more sensitive endpoint to C. elegans than others evaluated. MenosCaenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on... Mostrar Tudo |
Thesagro: |
Toxicologia. |
Thesaurus Nal: |
Caenorhabditis elegans. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161034/1/2016RA-093.pdf
|
Marc: |
LEADER 02255nam a2200157 a 4500 001 2071457 005 2017-07-10 008 2016 bl uuuu u00u1 u #d 100 1 $aCASTRO, V. L. S. S. de 245 $aEvaluation of NANoREG TiO2 toxicity in Caenorhabiditis elegans.$h[electronic resource] 260 $aIn: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B4TJ.$c2016 520 $aCaenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on the concentration tested, but also on the endpoint. Growth seems to be a more sensitive endpoint to C. elegans than others evaluated. 650 $aCaenorhabditis elegans 650 $aToxicologia 700 1 $aCASTANHA, R. F. 700 1 $aVALLIM, J. H.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
17/03/2009 |
Data da última atualização: |
23/09/2015 |
Autoria: |
VIEIRA NETO, J.; OLIVEIRA A. F. de; OLIVEIRA, N. C. de; DUARTE, H. da S. S.; GONÇALVES, E. D. |
Título: |
Aspectos técnicos da cultura da oliveira. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Belo Horizonte: EPAMIG, 2008. |
Páginas: |
56 p. |
Série: |
(EPAMIG. Boletim técnico, 88). |
Idioma: |
Português |
Conteúdo: |
Comparação atural entre a fruticultrua e a olivicultura no mundo e no Brasil; Botânica e descrição da planta; Recomendações para implantação e olivais; Tratos culturais; Colheita; Como preparar conserva de azeitonas; Azeite; Coeficientes técnicos. |
Palavras-Chave: |
Cultivo; Oliveira. |
Thesagro: |
Azeite; Azeitona. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00838nam a2200229 a 4500 001 1427338 005 2015-09-23 008 2008 bl uuuu u0uu1 u #d 100 1 $aVIEIRA NETO, J. 245 $aAspectos técnicos da cultura da oliveira. 260 $aBelo Horizonte: EPAMIG$c2008 300 $a56 p. 490 $a(EPAMIG. Boletim técnico, 88). 520 $aComparação atural entre a fruticultrua e a olivicultura no mundo e no Brasil; Botânica e descrição da planta; Recomendações para implantação e olivais; Tratos culturais; Colheita; Como preparar conserva de azeitonas; Azeite; Coeficientes técnicos. 650 $aAzeite 650 $aAzeitona 653 $aCultivo 653 $aOliveira 700 1 $aOLIVEIRA A. F. de 700 1 $aOLIVEIRA, N. C. de 700 1 $aDUARTE, H. da S. S. 700 1 $aGONÇALVES, E. D.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|